Coding

Part:BBa_K643002:Design

Designed by: Rikki Frenkel, Justin Fabrikant   Group: iGEM11_Columbia-Cooper   (2011-09-28)


J140 metal binding peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.


Source

This protein was selected from a pIII phage display library based on its ability to nucleate ZnS or CdS nanocrystals.

References