Coding
Part:BBa_K643002:Design
Designed by: Rikki Frenkel, Justin Fabrikant Group: iGEM11_Columbia-Cooper (2011-09-28)
J140 metal binding peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 48
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.
Source
This protein was selected from a pIII phage display library based on its ability to nucleate ZnS or CdS nanocrystals.